The potential impact of PM2.5 on the covid-19 crisis in the Brazilian Amazon region

ABSTRACT OBJECTIVE This study aims to assess covid-19 morbidity, mortality, and severity from 2020 to 2021 in five Brazilian Amazon states with the highest records of wildfires. METHODS A distributed lag non-linear model was applied to estimate the potential exposure risk association with particulate matter smaller than 2.5-µm in diameter (PM2.5). Daily mean temperature, relative humidity, percentual of community mobility, number of hospital beds, days of the week, and holidays were considered in the final models for controlling the confounding factors. RESULTS The states of Para, Mato Grosso, and Amazonas have reported the highest values of overall cases, deaths, and severe cases of covid-19. The worrying growth in the percentual rates in 2020/2021 for the incidence, severity, and mortality were highlighted in Rondônia and Mato Grosso. The growth in 2020/2021 in the estimations of PM2.5 concentrations was higher in Mato Grosso, with an increase of 24.4%, followed by Rondônia (14.9%). CONCLUSION This study establishes an association between wildfire-generated PM2.5 and increasing covid-19 incidence, mortality, and severity within the studied area. The findings showed that the risk of covid-19 morbidity and mortality is nearly two times higher among individuals exposed to high concentrations of PM2.5. The attributable fraction to PM2.5 in the studied area represents an important role in the risk associated with covid-19 in the Brazilian Amazon region.

Multidrug-resistant Acinetobacter spp. from hospital intensive care units in Brazilian Amazon

Abstract Acinetobacter spp. are one of the main pathogens responsible for healthcare-associated infections and are associated with high rates of morbidity and mortality globally, mainly because of their high capacity to present and develop resistance to antimicrobials. To identify species of the Acinetobacter and their resistance profiles from samples collected from hospitalized patients, health professionals and hospital environmental sources in the intensive care units of different public reference hospitals in Porto Velho City, Rondônia, Western Brazilian Amazon. Isolates were identified using microbiological and molecular techniques. The antimicrobial susceptibility profile was determined by disk diffusion. A total of 201 Acinetobacter spp. isolates were identified, of which 47.3% originated from hospital structures, 46.8% from patients and 6% from healthcare professionals. A. baumannii and A. nosocomialis were the most prevalent, with frequency of 58.7% and 31.8%, respectively. Regarding the susceptibility profile, it was observed that 56.3% were classified as multidrug-resistant and 76.2% of the samples belonging to A. baumannii were resistant to carbapenems. In contrast, 96.9% were susceptible to polymyxin B and 91.3% to doxycycline. The data presented here can be used to guide and strengthen the control of multidrug-resistant infections caused by Acinetobacter spp., in addition to improving providing information from a traditionally unassisted region of Brazil.

Effect of weekly versus daily primaquine on Plasmodium vivax malaria recurrences: A real-life cohort study

ABSTRACT Background: Although primaquine (PQ) is indicated for G6PD-deficient patients, data on weekly PQ use in Brazil are limited. Methods: We aimed to investigate malaria recurrences among participants receiving daily and weekly PQ treatments in a real-life setting of two municipalities in the Amazon between 2019 and 2020. Results: Patients receiving weekly PQ treatment had a lower risk of recurrence than those receiving daily PQ treatment (risk ratio: 0.62, 95% confidence interval: 0.41-0.94), using a model adjusted for study site. Conclusions: Weekly PQ use did not increase the risk of malaria recurrence. Further studies with larger populations are warranted.

Prevalence of obesity, high blood pressure, dyslipidemia and their associated factors in children and adolescents in a municipality in the Brazilian Amazon region / Prevalência de obesidade, pressão arterial elevada e dislipidemia e seus fatores associados em crianças e adolescentes de um município amazônico, Brasil

INTRODUCTION: The incidence of chronic noncommunicable disease (CND) are rocketting over the world, including in young adults. The WHO estimates that more than half of the deaths in the world, even in underdeveloped countries, are caused by CND. OBJECTIVES: The study aimed to estimate the prevalence of obesity, high blood pressure (HBP) and dyslipidemia and its associated factors. METHODS: The authors carried out a cross-sectional study of 1,431 schools in the public-school system of Monte, Brazilian Western Amazon, with children and adolescents aged 6 to 15 years. A random sampling of 496 individuals was carried out. The OpenEpi platform was used to calculate the sample size, considering p<0.05 and a presumed prevalence of CND of 50%. The authors applied a clinical-epidemiological questionnaire, made anthropometric measurements and laboratory tests. Diagnostic parameters recommended by the recent guidelines of the Ministry of Health in Brazil were used. RESULTS: Prevalence of CND was: Obesity 11.8%, HBP of 6.7% and dyslipidemia of 25.4%. After multivariate log-binomial analysis of the dependent variables, the statistically significant risk factors were overweight 18.4%, sedentary lifestyle 32.2%, family history of cardiovascular disease 23.4%, family history of HBP 84.2%, family dyslipidemia 55.8%, family obesity 38.7% and family chronic renal disease 40.6%. CONCLUSION: The findings pointed out to a context with a relatively high prevalence of CND, as well as their associated factors. Intervention measures such as health education, food education, stimulation of physical exercise, better school feeding and an improvement of the public health system are needed to mitigate the occurrence of CND.

INTRODUÇÃO: A incidência de Doenças Crônicas Não Transmissíveis (DCNT) está aumentando em todo o mundo, inclusive em adultos jovens. A OMS estima que mais da metade das mortes no mundo, mesmo em países subdesenvolvidos, são causadas por DCNT. OBJETIVO: O estudo teve como objetivo estimar a prevalência de obesidade, pressão arterial elevada (PAE) e dislipidemia e seus fatores associados. MÉTODO: Os autores realizaram um estudo transversal com uma amostra randomizada de 496 de 1.431 alunos das escolas da rede pública de ensino de Monte Negro, Amazônia Ocidental, com crianças e adolescentes de 6 a 15 anos. Foi realizada uma amostragem aleatória de 496 indivíduos. Para o cálculo do tamanho da amostra foi utilizada a plataforma OpenEpi, considerando p <0,05 e prevalência presumida de DCNT de 50%. Os autores aplicaram um questionário clínico-epidemiológico, realizaram medidas antropométricas e exames laboratoriais. Foram utilizados parâmetros diagnósticos recomendados pelas diretrizes recentes do Ministério da Saúde do Brasil. Os dados foram analisados por por tetes estatísticos univariados e depois, multivariados, para se detectar associação entre causas e desfechos. RESULTADOS: A prevalência de DCNT foi: Obesidade 11,8%, Pressão Arterial Elevada de 6,7% e dislipidemia de 25,4%. Após análise log-binomial multivariada das variáveis dependentes, os fatores associados estatisticamente significativos foram sobrepeso 18,4%, sedentarismo 32,2%, história familiar de doença cardiovascular 23,4%, história familiar de hipertensão arterial sistêmica 84,2%, dislipidemia familiar 55,8%, obesidade familiar 38,7% e doença renal crônica familiar 40,6%. CONCLUSÃO: Os achados apontam para um contexto com prevalência relativamente elevada de DCNT, bem como seus fatores associados em crianças/adolescentes. Medidas de intervenção como educação em saúde, educação alimentar, estímulo à prática de exercícios físicos, melhor alimentação escolar e melhoria do sistema público de saúde são necessárias para mitigar a ocorrência de DCNT.

Scorpion species of medical importance in the Brazilian Amazon: a review to identify knowledge gaps

Scorpionism is a relevant medical condition in Brazil. It is responsible for most accidents involving venomous animals in the country, which leads to severe symptoms that can evolve to death. In recent years, an increase of almost 50% in the incidence of scorpionism has been observed in the Northern Region, where the highest severity of envenoming has been notified since the beginning of the 21st century. This review aims to provide an in-depth assessment of public data and reports on symptoms and epidemiology of envenoming, ecological aspects of scorpions, and characterization of venoms and toxins to access the gaps that need to be filled in the knowledge of the scorpion species of medical importance from the Brazilian Amazon. A systematic search using the string words "Amazon" and "scorpion" was performed on 11 databases. No restriction on date, language or status of the publication was applied. Reports not related to the Brazilian Amazon were excluded. Therefore, 88 studies remained. It is shown that populations of scorpions of medical importance, even of the same species, may present significant toxic variations peculiar to some regions in the Brazilian Amazon, and commercial scorpion antivenoms were not able to shorten the intensity and duration of neurological manifestations in patients stung by T. silvestris, T. apiacas or T. obscurus. It is also highlighted that the toxins responsible for triggering these alterations have not been elucidated yet and this is a fruitful field for the development of more efficient antivenoms. Furthermore, the geographic distribution of scorpions of the genus Tityus in the Brazilian Amazon was revised and updated. The cumulative and detailed information provided in this review may help physicians and scientists interested in scorpionism in the Brazilian Amazon.(AU)

Severe tissue complications in patients of Bothrops snakebite at a tertiary health unit in the Brazilian Amazon: clinical characteristics and associated factors

Abstract INTRODUCTION: Snakebites in the Brazilian Amazon are caused mostly by snakes from the Bothrops genus and envenomated patients may suffer from tissue complications. METHODS: This study aimed to identify risk factors for severe tissue complications (STC) in patients with Bothrops snakebite in the Amazonas state, Brazil. RESULTS: Snakebites that were classified as severe and affected female patients with comorbidities presented greater risks of developing STCs. In addition, hospitalizations of patients with STC exceeded 5 days. CONCLUSIONS: Clinical and epidemiological characteristics can prove essential for assessing the evolution of STC and clinical prognosis of patients with Bothrops snakebites.

Occurrence of anti-Toxoplasma gondii and anti-Neospora caninum antibodies in pigs in the State of Pará, Brazil / Ocorrência de anticorpos contra Toxoplasma gondii e Neospora caninum em suínos no estado do Pará, Brasil

Abstract We investigated the occurrence of Toxoplasma gondii and Neospora caninum antibodies in pigs raised in the Northeast of Pará, Brazil. At Study I, convenience sampled 151 pigs at two slaughterhouses, with and without state inspection; and Study II, which assessed 159 pigs with probabilistic sampling from nine pig farms. Serological analysis was performed using indirect fluorescent antibody test for T. gondii and N. caninum with a cutoff of 64 and 50, respectively. Overall, 6.77% pigs were seropositive for T. gondii and 5.16% for N. caninum. In Study I, pigs slaughtered with and without state inspection presented similar occurrence for both coccidia (p>0.05). Study II found an association between N. caninum seropositivity and sludge discarded into the soil, feeding pigs with animal-based protein, subsistence system, and absence of nipple drinkers. No association was found for T. gondii. Pigs from Pará are a potential source of T. gondii infection to humans. To our best knowledge, this is the first study to report anti-N. caninum antibodies in the serum of pigs in Pará State, Brazilian Amazon.

Resumo Foi investigada a ocorrência de anticorpos contra Toxoplasma gondii e Neospora caninum em suínos criados no nordeste do Pará, Brasil. No Estudo I, foram amostrados 151 porcos em dois matadouros, com e sem inspeção estadual. O Estudo II avaliou 159 suínos com amostragem probabilística de nove granjas de suínos. Para sorologia, utilizou-se o teste de imunofluorescência indireta para T. gondii e N. caninum com ponto de corte de 1:64 e 1:50, respectivamente. No geral, 6,77% dos suínos foram soropositivos para T. gondii e 5,16% para N. caninum. No Estudo I, suínos abatidos em matadouros com e sem inspeção estadual apresentaram ocorrência semelhante para ambos os coccídios (p> 0,05). Os animais amostrados de Belém, Benevides, Marituba, Bujaru, Castanhal e Igarapé-Miri foram positivos para T. gondii, enquanto os soropositivos para N. caninum foram encontrados em Belém, Bujaru, Castanhal e Santo Antônio do Tauá. O Estudo II encontrou associação entre soropositividade de N. caninum e esterco descartado no solo, alimentação dos suínos com proteína de origem animal, criação de subsistência e ausência de bebedores tipo "nipple". Não foi encontrada associação para T. gondii. A carne suína apresenta potencial risco de transmissão de T. gondii para os habitantes da região. De acordo com nosso conhecimento, este é o primeiro relato de anticorpos anti-N. caninum em suínos no estado do Pará, Amazônia brasileira.

Temporal distribution of fruit-feeding butterflies (Lepidoptera, Nymphalidae) in the eastern extreme of the Amazon region

Rainfall is one of the most influential factors driving insect seasonality in the Amazon region. However, few studies have analyzed the temporal dynamics of fruit-feeding butterflies in the Brazilian Amazon, specially in its eastern portion. Here, we evaluated the diversity patterns and temporal distribution of fruit-feeding butterflies in a remnant of eastern Amazon forest in the Baixada Maranhense, northeastern Brazil. Specifically, we tested whether fruit-feeding butterflies are temporally structured and whether rainfall influences species richness and abundance. Butterflies were collected with baited traps in both the rainy and dry seasons for two consecutive years. In total, we captured 493 butterflies belonging to 28 species, 15 genera and eight tribes. Three species comprised about half of the overall abundance, and Satyrinae was the most representative subfamily. The fruit-feeding butterfly assemblage showed a strong temporal structure during the second year of sampling, but not during the first year. Species composition and richness did not differ between rainy and dry seasons, and neither abundance nor richness was influenced by rainfall. Our results indicate that seasonality is not a strong environmental filter in this region, and that other biotic and abiotic factors are probably driving the community structure. The predominance of palms in the Baixada Maranhense, which are used as host plants by larvae of several lepidopteran species (specially satyrines) and are available year-round, might have contributed to the observed patterns of temporal diversity. (AU)

Do local environmental factors structure the trophic niche of the splash tetra, Copella arnoldi? A test in an Amazonian stream system

Environmental variation affects the availability of spatial and trophic resources in Amazonian streams and may be important factors structuring the diet of fishes. We analyzed the diet composition and trophic niche breadth of the lebiasinid splash tetra, Copella arnoldi, aiming to evaluate how environmental variation in Amazonian streams affects the species' trophic niche. Fish were captured and environmental factors were recorded in 20 streams in the Caxiuanã National Forest, in the eastern Amazon, in November 2010. We made a semi-quantitative analysis of stomach contents of 200 individuals. Copella arnoldi exhibited an omnivorous diet composed mainly of detritus and allochthonous invertebrates. Environmental variation (stream width, stream depth, canopy cover and flow) did not affect the diet composition or trophic niche breadth of the species, possibly due to the regional integrity of the forest within the boundaries of the protected area. Riparian cover probably minimizes the effect of the small-scale variations in food resources, thus leading to a locally homogeneous diet composition in the splash tetra C. arnoldi. (AU)

Community-based approaches for malaria case management in remote communities in the Brazilian Amazon

Abstract INTRODUCTION Malaria case management is a pivotal intervention in malaria elimination. However, many remote areas in Brazil still lack access to basic health services. This study describes a community-based approach (CBA) for malaria case management in the large remote area of the Jaú National Park (JNP), Amazonas, Brazil. METHODS In 2001, a general health CBA was initiated with a motor group (MG); a participative community health diagnosis (PCHD) was subsequently implemented between 2001 and 2005. In 2006, a CBA for malaria case management started with an expanded MG including all sectors with a stake in malaria control, from the local residents to the federal government. In 2008, community microscopists were selected and trained to diagnose hemoparasites. A full malaria strategy was implemented in 2009 with subsequent quality control follow-up. RESULTS Two educational materials were co-created with local communities. The MG identified malaria as a major health problem and the malaria MG planned the control activities. Ten communities selected a resident to become malaria microscopists, and ten solar-operated health centers were built. The number of slide readings increased from 923 in 2006 to 1,900 in 2009, while malaria infections decreased from 354 cases in 2005 to 20 cases in 2015. The excess time (≥ 48 hours) between first symptoms and diagnosis/treatment decreased from 68.9% of cases in 2005 to 14.3% in 2010. CONCLUSIONS While many factors were likely involved in the reduction of malaria transmission in the JNP, the CBA played an important role in the sustained success of the initiative.

Health outcomes of the bolsa família program among Brazilian Amazonian children

ABSTRACT OBJECTIVE One of the primary objectives of Brazil's conditional cash transfer program, Bolsa Família, is to break the intergenerational transmission of poverty by improving human capital via conditionalities. In this study, we hypothesized that health indicators of Bolsa Família participants would be comparable to those of other local children who were nonparticipants after two years of follow-up in the city of Acrelândia, Acre state, Western Brazilian Amazon. METHODS Data from a population-based longitudinal study were analyzed to examine school enrollment, vaccination coverage, height and body mass index for age z-scores, and biomarkers of micronutrient deficiencies (iron and vitamin A) between Bolsa Família participants (n = 325) and nonparticipants (n = 738). RESULTS Out of 1063 children 10 years and younger included in the 2007 baseline survey, 805 had anthropometric measurements and 402 had biochemical indicators in the 2009 follow-up survey. Prevalence rate ratio (PRR) for non-enrollment in school at 4 years of age was 0.58 (95%CI: 0.34-1.02) when comparing Bolsa Família participants with nonparticipants. No difference was found for vaccination coverage, which was insufficient for most vaccine-preventable diseases. Bolsa Família participants were less likely to show a positive change in body mass index for age z-scores compared with nonparticipants (PRR = 0.81, 95%CI: 0.70-0.95), while a positive change in height for age z-scores was similar in the groups. No differences in micronutrient deficiencies were found between groups after 2 years. CONCLUSIONS Early school enrollment and consistent nutritional indicators between Bolsa Família participants and nonparticipants suggest Bolsa Família was facilitating similarities between groups over time.

First record of Thaumastocoris peregrinus (Hemiptera, Thaumastocoridae) in Pará state, Brazil

Thaumastocoris peregrinus (Hemiptera, Thaumastocoridae) is a phytophagous pest of eucalyptus plantations. It was introduced in Brazil in 2008, and expanded rapidly due to its adaptive capacity to different climatic conditions. Eucalyptus has been planted in the Brazilian Amazon since the 1980s, being used in silvopastoral systems and for cellulose production. Since its introduction in the southernmost Brazilian state of Rio Grande do Sul, T. peregrinus has expanded its geographical distribution to 117 municipalities in states in the south (Rio Grande do Sul, Santa Catarina and Paraná), southeast (São Paulo, Espirito Santo, Rio de Janeiro and Minas Gerais), mid-west (Mato Grosso do Sul and Goiás) and northeast (Sergipe) of the country. Here we report the first record of T. peregrinus in the Brazilian Amazonian state of Pará. (AU)

Dirofilaria immitis infection in dogs in Algodoal Island, Brazilian Amazon / Infecção por Dirofilaria immitis em cães na Ilha de Algodoal, Amazônia brasileira

Dirofilaria immitis, a parasite that mainly infects domestic or wild canids, but can infect felines or humans as well, is frequent in many Brazilian areas. The main objective of this research was to determine the prevalence of natural canine infection at the Algodoal-Maiandeua Island complex, in the coastal region of the state of Pará, Brazil. A total of 67 dogs were sampled for blood microfilariae detection and for D. immitis DNA detection. Microfilaria and D. immitis DNA could be detected in 35.8% (24/67) of the animals. In one dog's sample no microfilariae were detected, but the PCR was positive, suggesting that either larvae recently were eliminated or adults died shortly before sample collecting. Therefore, it can be concluded that the occurrence of D. immitis is a health threat for domestic and wild canids at the Island of Algodoal, as well as for feline or human health.(AU)

Dirofilaria immitis, um parasito que infecta principalmente canídeos domésticos ou selvagens, embora também possa infectar felinos e humanos, é frequente em muitas áreas do Brasil. O objetivo deste estudo foi determinar a ocorrência da infecção natural em cães provenientes do complexo da Ilha de Algodoal-Maiandeua, região litorânea do estado do Pará, Brasil. Um total de 67 cães tiveram o sangue coletado para detecção de microfilárias de D. immitis e seu DNA. Microfilárias e o DNA de D. immitis foram detectados em 35,8% (24/67) dos animais. Na amostra de um animal, não foram observadas microfilárias, mas o seu DNA foi detectado, sugerindo que as larvas tenham sido recentemente eliminadas ou os adultos tenham morrido antes da coleta da amostra. Portanto, pode-se concluir que a ocorrência de D. immitis é uma ameaça à saúde de canídeos domésticos no complexo da Ilha de Algodoal-Maiandeua, bem como para felinos e seres humanos.(AU)

Relevance of wood anatomy and size of Amazonian trees in the determination and allometry of sapwood area

Hydrological processes in forest stands are mainly influenced by tree species composition and morpho-physiological characteristics. Few studies on anatomical patterns that govern plant hydraulics were conducted in tropical forest ecosystems. Thus, we used dye immersion to analyze sapwood area patterns of 34 trees belonging to 26 species from a terra firme forest in the central Brazilian Amazon. The sapwood area was related with wood anatomy and tree size parameters (diameter-at-breast-height - DBH, total height and estimated whole-tree volume). Exponential allometric equations were used to model sapwood area using the biometrical variables measured. Sapwood area traits (cross-section non-uniformity and heartwood visibility) varied significantly among and within species even though all were classified as diffuse porous. DBH was strongly and non-linearly correlated with sapwood area (R 2 = 0.46, P < 0.001), while no correlation was observed with vessel-lumen diameter (P = 0.94) and frequency (P = 0.58). Sapwood area and shape were also affected by the occurrence of vessel obstruction (i.e., tyloses), hollow stems and diseases. Our results suggest that sapwood area patterns and correlated variables are driven by intrinsic species characteristics, microclimate and ecological succession within the stand. We believe that individual tree sapwood characteristics have strong implications over water use, hydrological stand upsaling and biomass quantification. These characteristics should be taken into account (e.g., through a multi-point sampling approach) when estimating forest stand transpiration in a highly biodiverse ecosystem. (AU)

Processos hidrológicos de povoamentos florestais são predominantemente influenciados pela composição de espécies arbóreas e suas características morfo-fisiológicas. No entanto, existem poucos estudos sobre os padrões anatômicos que determinam o sistema hidráulico de plantas em ecossistemas tropicais. Por isso, nosso objetivo foi o de analisar os padrões da área do xilema ativo em 34 árvores de 26 espécies de uma floresta de terra firme na Amazônia central por meio de imersão em solução de corante. A área do xilema ativo foi relacionada a características autoecológicas das espécies, anatomia da madeira e parâmetros de crescimento (diametro à altura do peito - DAP, altura total e volume total). Equações alométricas exponenciais foram utilizadas para ajustar a área do xilema às variáveis medidas. Características do alburno (área transversal não-uniforme e visibilidade do cerne) variaram significativamente entre e dentro de espécies, apesar de que todas as espécies apresentaram vasos difusos. DAP foi fortemente e não-linearmente correlacionado à área do alburno (R 2 = 0,46; P < 0,001), enquanto diâmetro (P = 0,94) e frequência (P = 0.58) de vasos não apresentaram nenhum grau de relacionamento. O tamanho e forma do alburno foram afetados pela ocorrência de obstrução de poros (tilose) e troncos ocos. Estes padrões sugerem que a área do xilema é influenciada por características intrínsicas de cada espécie, microclima e estágio sucessional dentro do povoamento. Nossos resultados implicam que características individuais de árvores podem fortemente influenciar o transporte de água e, consequentemente, os processos hidrológicos e a quantificação de biomassa do povoamento. Essas caracteristicas deveriam ser consideradas (por exemplo, por meio da coleta de amostras da área do xilema ativo ao longo da área transversal) ao estimar-se a transpiração de uma floresta altamente biodiversa.(AU)

Type 1 cardiorenal syndrome in a patient with an acute infection caused by Trypanosoma cruzi in the Brazilian Amazon region - a case report

Abstract Cardiorenal syndrome type 1 (CRS 1) occurs when acute heart failure leads to acute kidney injury. There are several etiologies of CRS 1, including Chagas disease. Here, we present the first case report of CRS 1 in a patient with acute Chagas disease. Electrocardiography, transthoracic echocardiography, and cardiac magnetic resonance imaging showed signs of acute myocarditis. Laboratory examination revealed severe loss of kidney function, with a creatinine clearance of 30 mL/min, which fully normalized after treatment. Due to emergence of Chagas disease in the Brazilian Amazon, it is important to report unique clinical features in order to improve patients' outcomes.

Surveillance of seroepidemiology and morbidity of Chagas disease in the Negro River, Brazilian Amazon

BACKGROUND Chagas disease in the Brazilian Amazon Region was previously regarded as an enzootic disease of wild animals. More recently, in situations where humans have penetrated the wild ecotope or where triatomines and/or wild animals (marsupials) have invaded human homes resulting in disease transmission, Chagas disease has come to be regarded as an anthropozoonosis. We found that the highest incidence of infection due to Trypanosoma cruzi and Chagas disease occurred among piassaba fibre gatherers and their families. OBJECTIVES Considering the results of previous surveys, we conducted a new survey of piassaba gatherers and their families in the creeks of the Aracá, Curuduri, Demini, Ererê and Padauiri rivers, which are tributaries on the left bank of the Negro River, in the municipality of Barcelos; Barcelos-Caurés highway; Negro River in Santa Isabel of the Negro River; and Marié River, on the right bank of the Negro River. METHODS A questionnaire was applied to 482 piassaba gatherers and their families who accompanied them. We collected 5-mL blood samples (with permission from each subject), separated the serum, and performed serological tests using indirect immunofluorescence and conventional and recombinant enzyme-linked immunosorbent assays (ELISA). We performed brief clinical examination and electrocardiograms. Only 273 subjects attended our field base for detailed clinical examination and electrocardiogram. FINDINGS AND MAIN CONCLUSIONS The questionnaire revealed that 100% of the 482 patients recognised the triatomine Rhodnius brethesi, which they had seen in the piassaba plantation and 81% in their field huts. A total of 79% of subjects had previously been bitten by this vector and 21% did not know. The 25 subjects seropositive for T. cruzi infection (5.2%) stated that they had been bitten more than 10 times by this insect. Of the 273 subjects who underwent electrocardiogram, 22% showed conditions that were possibly attributable to Chagas disease or other cardiovascular disease.

First record of acerola weevil, Anthonomus tomentosus (Faust, 1894) (Coleoptera: Curculionidae), in Brazil / Primeiro registro do bicudo dos frutos da acerola, Anthonomus tomentosus (Faust, 1894) (Coleoptera: Curculionidae), no Brasil

Abstract The weevil of acerola fruits, Anthonomus tomentosus (Faust, 1894) (Coleoptera: Curculionidae), is recorded for the first time in Brazil. Samples of this insect were collected in fruits of acerola, Malpighia emarginata D.C. (Malpighiaceae), in four municipalities in the north-central region of Roraima State, in the Brazilian Amazon. Information about injuries observed in fruits infested with A. tomentosus, its distribution in Roraima, and suggestions for pest management are presented.

Resumo O bicudo dos frutos da acerola, Anthonomus tomentosus (Faust, 1894) (Coleoptera: Curculionidae), é registrado pela primeira vez no Brasil. Amostras deste inseto foram coletadas em frutos de acerola, Malpighia emarginata D.C. (Malpighiaceae), em quatro municípios do Centro-Norte do Estado de Roraima, na Amazônia brasileira. Informações sobre as injúrias observadas nos frutos infestados por A. tomentosus, sua distribuição em Roraima e sugestões para o seu manejo são apresentadas.

Use and traditional knowledge of Byrsonima crassifolia and B. coccolobifolia (Malpighiaceae) in a Makuxi community of the Roraima savanna, northern Brazil / Uso e conhecimento tradicional de Byrsonima crassifolia e B. coccolobifolia (Malpighiaceae) em uma Comunidade Makuxi na Savana de Roraima, extremo norte do Brasil

ABSTRACT Byrsonima crassifolia and B. coccolobifolia, popularly known as mirixis, muricis, mantecos or nances, are common fruit species in the Amazonian savannas. Their fruits are used in the preparation of juices and other beverages, while the other parts of the plants are used for different purposes in some indigenous communities. The aim of the present ethnobotanical study was to investigate the knowledge and traditional uses of mirixis in the Darora Indigenous Community, of the Makuxi ethnicity, in the São Marcos Indigenous Land, state of Roraima, northern Brazil. The knowledge on these species among the residents was analyzed considering their gender and age. A survey was carried out by means of semi-structured interviews with 60 respondents (36 men and 24 women), between 18 and 84 years of age. Data from the interviewees was compared between genders and two age groups (< 40 and ≥ 40 years of age). Ethnobotanical indices (Informant diversity value; Informant equability value) were calculated, and no significant differences were found in the diversity of uses between genders (U = 283.0000, p > 0.05) and age groups (U = 359.5000, p > 0.05), indicating that the knowledge on the species is evenly distributed among residents. Results show that both species are used for different purposes in the food, fuelwood and medicinal categories in the Darora Community, and this knowledge is widely shared among adult men and women of all ages.

RESUMO Byrsonima crassifolia e B. coccolobifolia, popularmente conhecidas como mirixis, muricis, mantecos ou nances, são espécies de frutíferas comuns que ocorrem nas savanas amazônicas. Os seus frutos são utilizados na preparação de sucos e outras bebidas, enquanto outras partes das plantas são utilizadas para diferentes fins em algumas comunidades indígenas. O objetivo do presente estudo etnobotânico foi investigar o conhecimento e usos tradicionais dos mirixis na Comunidade Indígena Darora, da etnia Makuxi, na Terra Indígena São Marcos, no estado de Roraima. Este trabalho analisou o conhecimento dessas espécies entre os moradores da comunidade. A pesquisa foi realizada por meio de entrevistas semi-estruturadas com 60 participantes (36 homens e 24 mulheres), entre 18 e 84 anos de idade. Os dados das entrevistas foram comparados entre gêneros e dois grupos de idade (< 40 anos de idade e ≥ 40 anos de idade). Índices etnobotânicos (Valor de diversidade do informante; Valor de equabilidade do informante) foram calculados, e não houve diferenças significativas na diversidade de usos entre os gêneros (U = 283,0000, p > 0,05) e grupos de idade (U = 359,5000, p > 0,05), indicando que o conhecimento das espécies é uniformemente distribuído entre os moradores. Os resultados mostraram que ambas as espécies são usadas com finalidades nas categorias alimentícia, combustível e medicinal na Comunidade Darora, e o conhecimento é amplamente compartilhado entre homens e mulheres, independentemente da faixa etária.

Composição demográfica domiciliar e dinâmica do uso do solo em Machadinho d'Oeste, Rondônia: evidências baseadas nos estágios iniciais da fronteira / Household demographic composition and land use dynamics in Machadinho d'Oeste, Rondônia: evidence from the frontier onset / Composición demográfica domiciliaria y dinámica del uso del suelo en Machadinho d'Oeste, Rondônia: evidencias basadas en las etapas iniciales de la frontera

Vários estudos sugerem a importância da mudança na composição demográfica domiciliar sobre a demanda e o uso da terra em fronteiras agrícolas. A maioria desses estudos, no entanto, analisa essa relação em estágios mais avançados do desenvolvimento das fronteiras, produzindo evidências conflitantes sobre o papel dos determinantes populacionais. Este artigo examina os efeitos da composição demográfica domiciliar (ciclo de vida domiciliar) e do tempo na propriedade (ciclo no lote) sobre a dinâmica de uso do solo em pequenas propriedades rurais em Machadinho d'Oeste, Rondônia. Foram utilizados dados de uma pesquisa censitária sobre os colonos do projeto de assentamento original, para 1987 e 1995. A relação entre os ciclos e as classes de uso do solo foi analisada por meio de modelos de regressão Beta e de Dirichlet. Observou-se uma relação consistente entre oferta de trabalho familiar e estoque desmatado no nível da propriedade rural. O tempo na propriedade também se mostrou associado ao desmatamento, embora seu efeito não linear sugira a experimentação com o ambiente biofísico local. Sugere-se que os efeitos do ciclo de vida e no lote se autodeterminam nos estágios iniciais da fronteira, indicando a importância dos efeitos da composição demográfica sobre a dinâmica de uso da terra.

Previous studies have found evidence of the importance of changes in household composition to explain land use choices in agricultural frontiers. Most of these studies, however, analyze these dynamics at later stages of the frontier development, rendering mixed evidence on the role of population drivers. This study examines the effects of household demographic composition (household life cycle) and time on property (property life cycle) on land use dynamics in small farms at Machadinho d'Oeste, Rondônia. Based on a unique dataset covering the agricultural frontier since its onset, the study analyzes these effects when their association with land cover dynamics is most likely. Data collected in 1987 and 1995 for a census survey regarding settlers on the original settlement area was used for the study. The relationship between cycles and types of land use was analyzed and modelled by means of Beta and Dirichlet regression models. A consistent relation between family labor supply and deforested stock was observed with regards to rural property. Time on the property was also associated with deforestation, although its non-linear effect suggests experimentation with the local biophysical environment. The effect of the life cycle and of the lot are self-determined in the initial stages of frontier development, indicating the relevance of the demographic dynamics of households for land use choices in agricultural frontiers.

Varios estudios sugieren la importancia de los cambios en la composición demográfica domiciliaria sobre la demanda y el uso de la tierra en fronteras agrícolas. La mayoría de esos estudios analiza sin embargo esa relación en etapas más avanzadas del desarrollo de las fronteras, y han producido evidencias conflictivas sobre el papel de los determinantes poblacionales. Este artículo examina los efectos de la composición demográfica del hogar (ciclo de vida domiciliaria) y del tiempo en la propiedad (ciclo en el lote) sobre la dinámica del uso del suelo en pequeñas propiedades rurales en Machadinho d'Oeste, Rondônia. Para ello fueron utilizados datos censales sobre los colonos del proyecto de asentamiento original para los años 1987 y 1995. La relación entre los ciclos y los tipos de uso del suelo fue analizada por medio de modelos de regresión Beta y de Dirichlet. Se observó una relación consistente entre la oferta de trabajo familiar y el stock deforestado en el nivel de la propiedad rural. El tiempo en la propiedad también aparece asociado a la deforestación, aunque su efecto no linear sugiera la experimentación con el ambiente biofísico local. Se sugiere que el efecto del ciclo de vida y en el lote se determina en las etapas iniciales de la frontera, indicando la importancia de los efectos de la composición demográfica sobre la dinámica del uso de la tierra.

Allergic dermatitis caused by Culicoides in Texel sheep in the state of Pará, Brazil / Dermatite alérgica causada por Culicoides em ovinos Texel no estado do Pará

Allergic dermatitis was studied at a farm located in the municipality of Castanhal in the state of Pará, Brazil, from December 2009 to December 2012. Clinical and epidemiological data were obtained during monthly visits to the farm. Skin biopsies were performed for histopathological examinations, and insects were captured with Centers for Disease Control and Prevention (CDC) traps and live-bait entomological vacuum devices. The property housed a herd of both genders, with 45 Santa Inês sheep of different ages and 72 Texel adults. Of the 72 Texel sheep, 56 were affected. Of these, 24 exhibited alopecia, erythema, edema, and crust in the ears, on top of the head, and less often, around the eyes. In addition to the lesions described above, 14 animals exhibited hyperpigmentation of the ears and/or around the eyes and crust in the nose. Another 18 animals exhibited chronic lesions characterized by deformed and thickened ears, alopecia with hyperpigmentation, and in most cases, secondary bacterial infections with abscesses and/or myiasis. Some of these animals had lost part of their ears. Histologically, the lesions were characterized as orthokeratotic hyperkeratosis, acanthosis, vacuolization, and necrosis of epidermal cells. An inflammatory infiltrate was present in the dermis, which was composed mainly of eosinophils and macrophages, some lymphocytes and mast cells, and few neutrophils, in addition to edema and collagen deposition. The Santa Inês sheep were not affected. Among the captured insects, 294 specimens of Culicoides (Hoffmania) plaumanni Spinelli, 57 specimens of C. (Hoffmania) insignis Lutz, and 27 specimens of other Culicoides species were identified. We conclude that C. plaumanni, C. insignis, and possibly other Culicoides species are involved in the etiology of allergic dermatitis in Texel sheep in the state of Pará. Santa Inês sheep in the same region and under the same conditions were not affected, which suggests lower susceptibility of these animals.(AU)

A doença foi estudada em uma propriedade localizada no município de Castanhal, Pará, no período de dezembro de 2009 a dezembro de 2012. A propriedade possuía um plantel de 117 ovinos, 45 da raça Santa Inês e 72 Texel. Dos 72 ovinos da raça Texel, 56 estavam afetados. Os principais sinais clínicos observados foram alopecia, eritema, edema, hiperpigmentação e crostas nas orelhas, parte superior da cabeça e ao redor dos olhos. Em alguns animais as orelhas estavam deformadas, espessadas e com abscessos e/ou miíase. Histologicamente, as lesões caracterizavam-se por hiperqueratose ortoqueratótica, acantose, vacuolização e necrose das células da epiderme. Na derme havia infiltrado inflamatório composto principalmente por eosinófilos e macrófagos, edema e deposição de colágeno. Entre os insetos capturados foram identificados 294 exemplares de Culicoides (Hoffmania) plaumanni Spinelli, 57 de Culicoides (Hoffmania) insignis Lutz e 27 exemplares pertencentes a outras espécies de Culicoides. Conclui-se que C. plaumanni, C. insignis e, possivelmente, outras espécies de Culicoides estejam envolvidos na etiologia da dermatite alérgica nos ovinos da raça Texel no estado do Pará. Ovinos da raça Santa Inês que se encontravam na mesma área e nas mesmas condições não foram afetados, sugerindo menor susceptibilidade.(AU)